Bioactive Secondary Metabolites and Free Radical Scavenging Potential of Endophytic Fungi Associated With Elaeocarpus Recurvatus Corner

Abstract

Endophytic fungi have recently gained attention due to their possible ability to produce bioactive compounds in nature. Recent studies have found several antioxidant compounds in the many secondary metabolites produced by endophytic fungus. Genetic engineering has the potential to increase the production of these beneficial antioxidant chemicals by these organisms, whether they are unicellular or micro-multicellular. These substances can enhance energy, health, and a variety of biotechnological applications. From Elaeocarpus recurvatus, endophytic fungi (Daldinia grandis, Daldinia loculata, and Aspergillus fumigatus) were isolated. The study evaluates Elaeocarpus recurvatus ethyl acetate secondary metabolites, in vitro antioxidant properties. The findings showed that the Daldinia loculata ethyl acetate extract contained substantial amounts of flavonoids (172.01 mg RE/g), tannin (50.35 mg GAE/g), and total phenols (82.63 mg GAE/g). Good resistance to DPPH (61.83 μg/L), ABTS (101527.8 μM TE/g extracts), superoxide (28.66%), and FRAP (205.18 mM Fe(II)E /mg Extract) was demonstrated by Daldinia loculata ethyl acetate extract. These results indicate that Elaeocarpus recurvatus leaves of the endophytic fungus Daldinia loculata, in particular the ethyl acetate extract, constitute a substantial antioxidant source with great potential.