To Investigate the Potential Diuretic Effect of Securinega Leucopyrus Leaf Extract in Vivo Using Experimental Animal Models and Determine its Comparative Efficacy with Standard Diuretics

Abstract

Diuretics are agents that promote urine production by increasing the excretion of water and electrolytes from the body and are widely used to manage conditions such as hypertension, edema, and heart failure. The present study evaluated the diuretic activity of the aqueous leaf extract of Securinega leucopyrus using an in vivo experimental model. Preliminary phytochemical screening and spectroscopic analyses (UV–Visible and FTIR) confirmed the presence of bioactive compounds such as alkaloids, flavonoids, tannins, saponins, terpenoids, and polyphenols, which are known to possess therapeutic properties. The diuretic activity was assessed in Wistar rats at doses of 100, 200, and 400 mg/kg and compared with the standard diuretic drug furosemide (10 mg/kg). The extract produced a dose-dependent increase in urine output and significantly enhanced the excretion of sodium, potassium, and chloride ions. The highest dose showed diuretic activity comparable to the standard drug. These findings suggest that Securinega leucopyrus possesses significant diuretic potential and may serve as a promising natural therapeutic agent.